t4 dna ligase (New England Biolabs)

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New England Biolabs t4 dna ligase
T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33344 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t4+dna+ligase/T4+DNA+Ligase/pmc12996642-80-33-36
Average 99 stars, based on 33344 article reviews

t4 dna ligase - by Bioz Stars, 2026-09

99/100 stars

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Construct:

Article Title: A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling
Article Snippet: .. Vectors were pretreated with Antarctic phosphatase (M0289, NEB), and the DVL, CK1ε, or CK1δ constructs were ligated using T4 DNA Ligase (M0202, NEB). ..

Ligation:

Article Title: On-oligonucleotide olefin metathesis in water.
Article Snippet: .. For ligation (40 μL scale), 100 pmol of each oligonucleotide, 600 units of T4 DNA Ligase (T4 DNA ligase, New England Biolabs) and 1x T4 DNA Ligase Buffer (50 mM TrisHCl, 10 mM MgCl2, 10 mM DTT, 1 mM ATP, pH = 7.5 at 25 °C, New England Biolabs) were mixed. ..

Article Title: Generation of precise and accurate engineered circRNAs using enzymatic ligation
Article Snippet: .. 2000 units of T4 DNA ligase (NEB #M0202M) were used for ligation at 37°C for 1 h. RNA ligase 2 (NEB #M0239L) reactions were either set up with DNA splint or RNA splint. .. Reaction conditions remained the same for both the splints: 15 units of enzyme with 10% polyethylene gylcol (PEG), 100 mM MgCl 2 , and RNA ligase 2 buffer, and reactions were incubated at 25°C for 1 h. For RNA ligase 1 (NEB #M0204S) reactions, 25 units of the enzyme were added with 10% PEG, 1× RNA ligase buffer and incubated at 37°C for 1 h. When DNA splints were included, the addition of 2 units of Turbo DNase (Invitrogen #AM2238) for 15 min at 37°C degraded the DNA immediately after the end of ligation incubation.

Article Title: Longitudinal Characterization of Gingival Microbiota Following Periodontally Accelerated Osteogenic Orthodontics Surgery in Skeletal Class III Orthognathic Patients
Article Snippet: DNA (1 pg-200 ng) was digested with 4 U of BcgI (NEB) for 3 hours at 37 °C. .. Adaptors were then ligated to the DNA fragments by mixing 5 μl of digested DNA with 10 μl of a ligation master mix containing 0.2 μM of each adaptor and 800 U of T4 DNA ligase (NEB). ..

Plasmid Preparation:

Article Title: Novel ADPRS Missense Variant (p.Leu162Pro) Causes Stress-Induced Childhood-Onset Neurodegeneration With Ataxia and Seizures
Article Snippet: .. These oligonucleotides were annealed and inserted into eSpCas9(1.1) vector (addgene #71814) using restriction enzyme BbsI and T4 DNA ligase from NEB (MA). .. Competent Escherichia coli cells (NEB® 5-alpha strain, MA) were transformed by heat shock and cloned vectors were purified by the Monarch® Plasmid Miniprep Kit (NEB, MA). hTERT-RPE1 cells were then transfected with this vector by electroporation, using NeonTM Transfection System (Thermo Fisher, MA), along with vector pCD2E (a kind gift of K. Caldecott, Univ.

Purification:

Article Title: Poliovirus 3Dpol polymerase region is essential for cleavage of poliovirus 3AB in vivo.
Article Snippet: The RT-PCR products were purified using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio, #740609.250). .. T4 DNA ligase buffer (NEB, #B0202S) (3 μL) and T4 DNA ligase (NEB, #M0202L) (800 U, 2 μL) were added to the purified RT-PCR products (25 μL). .. The samples were incubated at room temperature for 2 h for circularization and then purified using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio, #740609.250).

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t4 dna ligase (New England Biolabs)

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